Amplification of CHD-1 gene fragment in Z and W sex chromosomes of Cemani chicken using a different set of PCR primers

Abstract Cemani chicken is a local breed of Indonesian known for its mystical appearance. Selecting sex earlier essential the breeding program to determine population structure. Instead using phenotype, sexing in birds can be done molecularly by employing variation CHD-1 gene, which belongs Z chromosome. Five primers (pair), namely AVP2 and AVP8; CHD1F CHD1R; AV1237L AV1272H; CHDZ CHDW, then AV2550F AV2718R, were employed amplify gene PCR reaction followed with electrophoresis analysis. blood was collected from Bracial vein, DNA extraction. Two set, CHD1R, able distinguish difference between male (ZZ) vs. female (ZW) chromosome showing two clear bands source sample one band sample. Meanwhile, AVP8, AV1237L, AV1272H, only show both samples, no resulted CHDW primer. sets fragment amplified further molecular determination chicken.